Michaela Trilck-Winkler
Michaela Trilck-Winkler, PhD
Postdoc, Head of Laboratory
Phone number:+49 451 3101 8203

Current Position:

Head of Laboratory and Postdoctoral Researcher (Section Applied Stem Cell Biology)
Project Coordinator of the Research Unit FOR2488 “Protect Move”

Research focus:

Functional studies in iPSC-derived neuronal cells generated from patients with Parkinson´s or NPC1 disease

Education & Scientific training

Education:

10/2005 – 09/2008 Studies of Medical Biotechnology (University of Rostock)
Bachelor Thesis “Characterization of the L- and D-lactate dehydrogenase from Streptococcus pyogenes” (Prof. Bernd Kreikemeyer, Institute for Medical Microbiology, Virology and Hygiene, Rostock)

10/2008-09/2010 Studies of Medical Biotechnology (University of Rostock)
Master Thesis „ Effects of ADAM10 on the Gene Expression in the Developing Chicken Spinal Cord “(Prof. Arndt Rolfs, Albrecht Kossel-Institute for Neuroregeneration, Rostock)

11/2010-12/2014 PhD Studies (Prof. Arndt Rolfs, Albrecht Kossel-Institute for Neuroregeneration, Rostock)
PhD Thesis “Generation, Characterization and Neural Differentiation of Human Induced Pluripotent Stem Cells – a Cellular Model for Niemann-Pick Type C1 Disease” (summa cum laude)

12/2014-06/2015 Postdoctoral Researcher at the Albrecht Kossel-Institute for Neuroregeneration, Rostock

07/2015-present Postdoctoral Researcher at the Institute of Neurogenetics, University of Luebeck

12/2016-present Project Coordinator of the Research Unit FOR2488 “Protect Move”

01/2018-present Laboratory Head Section “Applied Stem Cell Biology”


Advanced training:

11/2010 Workshop Pluripotent Stem Cells (ESHRE)

04/2011 FELASA B (compact course laboratory animals, animal testing and alternative methods)

10/2015 Quality Management Basics I (DGQ)

Supervision

2013 Participant in the DAAD RISE scholarship program (supervision of Ellen Körner)

Research Funding

2015 Career Development Grant FORUN from Rostock University Medical Center

2017-2018 Career Development Grant FuL from Medical Faculty of the University of Luebeck

Publications

Selection of 5 most important publications out of 7;
cumulative impact factor: 21; mean 3

  1. Trilck M, Hübner R, Seibler P, Klein C, Rolfs A, Frech MJ. Niemann-Pick type C1 patient-specific induced pluripotent stem cells display disease specific hallmarks. Orphanet J Rare Dis. 2013;8:144.

  2. Trilck M, Peter F, Zheng C, Frank M, Dobrenis K, Mascher H, Rolfs A, Frech MJ. Diversity of Glycosphingolipid GM2 and Cholesterol Accumulation in NPC1 Patient-Specific iPSC-Derived Neurons. Brain Res. 2017 Feb 15; 1657: 52-61. [Epub 2016 Dec 5]

  3. Zanon A, Kalvakuri S, Rakovic A, Foco L, Guida M, Schwienbacher C, Serafin A, Rudolph F, Trilck M, Grünewald A, Stanslowsky N, Wegner F, Giorgio V, Lavdas AA, Bodmer R, Pramstaller PP, Klein C, Hicks AA, Pichler I, Seibler P3. SLP-2 interacts with Parkin in mitochondria and prevents mitochondrial dysfunction in Parkin-deficient human iPSC-derived neurons and Drosophila. Hum Mol Genet. 2017 Jul 1;26(13):2412-2425. doi: 10.1093/hmg/ddx132.

  4. Rabenstein M, Peter F, Joost S, Trilck M, Rolfs A, Frech MJ. Decreased calcium flux in Niemann-Pick type C1 patient-specific iPSC-derived neurons due to higher amount of calcium-impermeable AMPA receptors. Mol Cell Neurosci. 2017 Jun 27;83:27-36. doi: 10.1016/j.mcn.2017.06.007. Epub 2017 Jun 27.

  5. Baumann H; Jahn M; Münchau A; Trilck-Winkler M*; Lohmann K*; Seibler P*. Generation and characterization of eight human-derived iPSC lines from affected and unaffected THAP1 mutation carriers. Stem Cell Res [under review]

Complete list of publications
  1. Trilck M, Hübner R, Seibler P, Klein C, Rolfs A, Frech MJ. Niemann-Pick type C1 patient-specific induced pluripotent stem cells display disease specific hallmarks. Orphanet J Rare Dis. 2013;8:144.

  2. Mußmann C, Hübner R, Trilck M, Rolfs A, Frech MJ. HES5 is a key mediator of Wnt-3a-induced neuronal differentiation. Stem Cells Dev. 2014;23(12):1328-39.

  3. Trilck M, Hübner R, Frech MJ. Generation and Neuronal Differentiation of Patient-Specific Induced Pluripotent Stem Cells Derived from Niemann-Pick Type C1 Fibroblasts. Methods Mol Biol. 2016;1353:233-59.

  4. Trilck M, Peter F, Zheng C, Frank M, Dobrenis K, Mascher H, Rolfs A, Frech MJ. Diversity of Glycosphingolipid GM2 and Cholesterol Accumulation in NPC1 Patient-Specific iPSC-Derived Neurons. Brain Res. 2017 Feb 15; 1657: 52-61. [Epub 2016 Dec 5]

  5. Peter F*, Trilck M*, Rabenstein M, Rolfs A, Frech MJ. Dataset in support of the generation of Niemann-Pick disease Type C1 patient-specific iPS cell lines carrying the novel NPC1 mutation c.1180T>C or the prevalent c.3182T>C mutation - Analysis of pluripotency and neuronal differentiation. Data Brief. 2017 Apr 2; 12: 123-131.

  6. Zanon A, Kalvakuri S, Rakovic A, Foco L, Guida M, Schwienbacher C, Serafin A, Rudolph F, Trilck M, Grünewald A, Stanslowsky N, Wegner F, Giorgio V, Lavdas AA, Bodmer R, Pramstaller PP, Klein C, Hicks AA, Pichler I, Seibler P3. SLP-2 interacts with Parkin in mitochondria and prevents mitochondrial dysfunction in Parkin-deficient human iPSC-derived neurons and Drosophila. Hum Mol Genet. 2017 Jul 1;26(13):2412-2425. doi: 10.1093/hmg/ddx132.

  7. Rabenstein M, Peter F, Joost S, Trilck M, Rolfs A, Frech MJ. Decreased calcium flux in Niemann-Pick type C1 patient-specific iPSC-derived neurons due to higher amount of calcium-impermeable AMPA receptors. Mol Cell Neurosci. 2017 Jun 27;83:27-36. doi: 10.1016/j.mcn.2017.06.007. Epub 2017 Jun 27.

  8. Trillhaase A, Haferkamp U, Rangnau A, Märtens M, Schmidt B, Trilck M, Seibler P, Aherrahrou R, Erdmann J, Aherrahrou Z. Differentiation of human iPSCs into VSMCs and generation of VSMC-derived calcifying vascular cells. Stem Cell Res. 2018 Jul 17;31:62-70. doi: 10.1016/j.scr.2018.07.008.m. Neur

  9. Baumann H; Jahn M; Münchau A; Trilck-Winkler M*; Lohmann K*; Seibler P*. Generation and characterization of eight human-derived iPSC lines from affected and unaffected THAP1 mutation carriers. Stem Cell Res [under review]

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